When is the rate the highest?
The experiment was performed two times. The average reaction slope is in column five and the standard deviation is noted in column six. Our original hypothesis was that as we decrease the amount of substrate reaction will speed up.
The slope of the line will get steeper. As we decreased the amount of substrate while the enzyme was kept constant resulted in a decrease of the reaction rate for the mixture.
The results of this experiment show me how there must be a balance between the amount of substrate and the amount of enzyme in a reaction. In our experiment the reaction rate did not increase, it decreased.
Because we reduced the amount of substrate in each mixture but kept the amount of enzyme the same the enzyme cannot react with what is not there. This is why the reaction rate of the mixture did not increase it decreased. Therefore the slope of the reaction declined as we reduced the amount of the substrate in each reaction.
Trial one and two did not produce the same reaction rate. This can be attributed to how the chemicals were measured for each mixture or the speed at which the stopper was inserted into the test tube.
Therefore the results were different in each trail. As a result I calculated the average reaction rate for each mixture and the standard deviation for each A1 vs. A2 and B1 vs B2 and so on. We did try to eliminate one aspect of human error by having the same person perform the same task in the experiment each time.
It is hard to pinpoint the exact element that result in the variations between trial one and trial two. Perhaps if we had more time, we could have performed the experiment more times to produce results that did not have such a large standard deviation.
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